Improvement of lupine breeding for alkaloid content in grain
Abstract
The paper presents the results of breeding of narrow-leaved, yellow and white lupine for alkaloid content in grain. It is shown that, when creating lupine varieties, one of the important monitored indicators is low alkaloid content. Due to the research it was established that the sum of 3 alkaloids (lupanine, sparteine, lupinine) fluctuated from 0.016 to 0.055 % with an average value of 0.027 % in the grain of breeding and collection samples of lupine species, including narrow-leaved lupine samples from 0.016 to 0.037 %, white - from 0.03 to 0.055 %, yellow - from 0.045 to 0.05 %. Improvement of traditional breeding methods for selecting lupine on low alkaloid content by using the marker analysis of genes, controlling the manifestation of the “seed alkaloid content” trait, made it possible to identify promising samples of narrow-leaved lupine.
About the Authors
M. N. Kritsky
RUE "Scientific and Practical Center for Agriculture of the National Academy of Sciences of Belarus"
Belarus
Belarus
Candidate of Agricultural Sciences
Zhodino
E. L. Dolgova
RUE "Scientific and Practical Center for Agriculture of the National Academy of Sciences of Belarus"
Belarus
Belarus
Candidate of Agricultural Sciences
Zhodino
N. V. Anisimova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Candidate of Biological Sciences
Minsk
A. A. Kozlovsky
RUE "Scientific and Practical Center for Agriculture of the National Academy of Sciences of Belarus"
Belarus
Belarus
Zhodino
I. V. Yushkevich
RUE "Scientific and Practical Center for Agriculture of the National Academy of Sciences of Belarus"
Belarus
Belarus
Zhodino
References
1. Alkaloid-analysis-in-lupins_FiBL_2024 [Electronic resource]: https://www. legumehub.eu/wp-content/uploads/2024/02/Alkaloid-analysis-in-lupins_FiBL_2024.pdf – Date of access: 14. 02. 2025.
2. Wink M. A summary of 30 years of research in lupins and lupin alkaloids. Lupin crops: an opportunity for today a promise for the future // Proc. 13<sup>th</sup> Int. Lupin Conf. Poznan, 2011. Pp. 225–228.
3. Руцкая, В. И., Тимошенко Е. С. Алкалоиды люпина и способы снижения их содержания. (Обзор). Известия НВ АУК. 2023. 3(71). 573-584. DOI: 10.32786/2071-9485-2023-03-56.
4. Li X., Yang H., Buirchell B., Yan G. Development of a DNA marker tightly linked to low-alkaloid gene iucundus in narrow-leafed lupin (Lupinus angustifolius L.). // Crop Past Sci, – 2011. – N 62. – P. 218–224.
5. Frick, K.M.; Foley, R.C.; Kamphuis, L.G.; Siddique, K.H.M.; Garg, G.; Singh, K.B. Characterization of the genetic factors affecting quinolizidine alkaloid biosynthesis and its response to abiotic stress in narrow-leafed lupin (Lupinus angustifolius L.). Plant Cell Environ. 2018, 41, 2155–2168.
6. Cowling, W.A.; Huyghe, C.; Swiecicki, W. Lupin breeding. In Lupins as Crop Plants: Biology, Production and Utilization; Gladstones, J., Atkins, C.A., Hamblin, J., Eds.; CAB International: London, UK, 1998; pp. 93–120.
7. Abraham E. M. The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches [Electronic resource] / Eleni M. Abraham, I. Ganopoulos, P. Madesis, A. Mavromatis [and others.] https://www.mdpi.
8. Bunsupa, S.; Yamazaki, M.; Saito, K. Quinolizidine alkaloid biosynthesis: Recent advances and future prospects. Front. Plant Sci. 2012, 3, 239.
9. Hane, J.K.; Ming, Y.; Kamphuis, L.G.; Nelson, M.N.; Garg, G.; Atkins, C.A.; Bayer, P.E.; Bravo, A.; Bringans, S.; Cannon, S.; et al. A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: Insights into plant-microbe interactions and legume evolution. Plant Biotechnol. J. 2017, 15, 318–330.
10. Nelson, M.N.; Moolhuijzen, P.M.; Boersma, J.G.; Chudy, M.; Lesniewska, K.; Bellgard, M.; Oliver, R.P.; Swiecicki, W.; Wolko, B.; Cowling, W.A.; et al. Aligning a new reference genetic map of Lupinus angustifolius with the genome sequence of the model legume, lotus japonicus. DNA Res. 2010, 17, 73–83.
Review
For citations:
Kritsky M.N., Dolgova E.L., Anisimova N.V., Kozlovsky A.A., Yushkevich I.V. Improvement of lupine breeding for alkaloid content in grain. Arable Farming and Plant Breeding in Belarus. 2025;(61):198-205. (In Russ.)
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