<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">earth</journal-id><journal-title-group><journal-title xml:lang="ru">Земледелие и селекция в Беларуси</journal-title><trans-title-group xml:lang="en"><trans-title>Arable Farming and Plant Breeding in Belarus</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0130-156X</issn><publisher><publisher-name>Научно-практический центр Национальной академии наук Беларуси по земледелию</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">earth-638</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СЕЛЕКЦИЯ И СЕМЕНОВОДСТВО</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>SELECTION AND SEED PRODUCTION</subject></subj-group></article-categories><title-group><article-title>Совершенствование методики селекции люпина по признаку «содержание алкалоидов в зерне»</article-title><trans-title-group xml:lang="en"><trans-title>Improvement of lupine breeding for alkaloid content in grain</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Крицкий</surname><given-names>М. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Kritsky</surname><given-names>M. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат с.-х. наук</p><p>Жодино</p></bio><bio xml:lang="en"><p>Candidate of Agricultural Sciences</p><p>Zhodino</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Долгова</surname><given-names>Е. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Dolgova</surname><given-names>E. L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат с.-х. наук</p><p>Жодино</p></bio><bio xml:lang="en"><p>Candidate of Agricultural Sciences</p><p>Zhodino</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Анисимова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Anisimova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук</p><p>Минск</p></bio><bio xml:lang="en"><p>Candidate of Biological Sciences</p><p>Minsk</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Козловский</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kozlovsky</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Жодино</p></bio><bio xml:lang="en"><p>Zhodino</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юшкевич</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Yushkevich</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Жодино</p></bio><bio xml:lang="en"><p>Zhodino</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>РУП «Научно-практический центр НАН Беларуси по земледелию»</institution><country>Беларусь</country></aff><aff xml:lang="en"><institution>RUE "Scientific and Practical Center for Agriculture of the National Academy of Sciences of Belarus"</institution><country>Belarus</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Институт генетики и цитологии НАН Беларуси</institution><country>Беларусь</country></aff><aff xml:lang="en"><institution>Institute of Genetics and Cytology of the National Academy of Sciences of Belarus</institution><country>Belarus</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>07</day><month>09</month><year>2025</year></pub-date><volume>0</volume><issue>61</issue><fpage>198</fpage><lpage>205</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Крицкий М.Н., Долгова Е.Л., Анисимова Н.В., Козловский А.А., Юшкевич И.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Крицкий М.Н., Долгова Е.Л., Анисимова Н.В., Козловский А.А., Юшкевич И.В.</copyright-holder><copyright-holder xml:lang="en">Kritsky M.N., Dolgova E.L., Anisimova N.V., Kozlovsky A.A., Yushkevich I.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://earth.belal.by/jour/article/view/638">https://earth.belal.by/jour/article/view/638</self-uri><abstract><p>   В статье приводятся результаты селекции люпина узколистного, желтого и белого по признаку содержание алкалоидов в зерне. Показано, что одним из важных контролируемых показателей при создании сортов люпина является низкое содержание алкалоидов. Исследованиями установлено, что в зерне у селекционных и коллекционных образцов видов люпина сумма 3 алкалоидов (люпанина, спартеина, люпинина) колебалась от 0,016 до 0,055 % при среднем значении 0,027 %, в том числе у образцов узколистного люпина от 0,016 до 0,037 %, белого – от 0,03 до 0,055 %, желтого – от 0,045 до 0,05 %. Совершенствование традиционных селекционных методик отбора люпина по низкой алкалоидности путем привлечения маркерного анализа генов, контролирующих проявление признака «алкалоидность семян», позволило выделить перспективные образцы люпина узколистного.</p></abstract><trans-abstract xml:lang="en"><p>   The paper presents the results of breeding of narrow-leaved, yellow and white lupine for alkaloid content in grain. It is shown that, when creating lupine varieties, one of the important monitored indicators is low alkaloid content. Due to the research it was established that the sum of 3 alkaloids (lupanine, sparteine, lupinine) fluctuated from 0.016 to 0.055 % with an average value of 0.027 % in the grain of breeding and collection samples of lupine species, including narrow-leaved lupine samples from 0.016 to 0.037 %, white - from 0.03 to 0.055 %, yellow - from 0.045 to 0.05 %. Improvement of traditional breeding methods for selecting lupine on low alkaloid content by using the marker analysis of genes, controlling the manifestation of the “seed alkaloid content” trait, made it possible to identify promising samples of narrow-leaved lupine.</p></trans-abstract></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Alkaloid-analysis-in-lupins_FiBL_2024 [Electronic resource]: https://www. legumehub.eu/wp-content/uploads/2024/02/Alkaloid-analysis-in-lupins_FiBL_2024.pdf – Date of access: 14. 02. 2025.</mixed-citation><mixed-citation xml:lang="en">Alkaloid-analysis-in-lupins_FiBL_2024 [Electronic resource]: https://www. legumehub.eu/wp-content/uploads/2024/02/Alkaloid-analysis-in-lupins_FiBL_2024.pdf – Date of access: 14. 02. 2025.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Wink M. A summary of 30 years of research in lupins and lupin alkaloids. Lupin crops: an opportunity for today a promise for the future // Proc. 13&lt;sup&gt;th&lt;/sup&gt; Int. Lupin Conf. Poznan, 2011. Pp. 225–228.</mixed-citation><mixed-citation xml:lang="en">Wink M. A summary of 30 years of research in lupins and lupin alkaloids. Lupin crops: an opportunity for today a promise for the future // Proc. 13&lt;sup&gt;th&lt;/sup&gt; Int. Lupin Conf. Poznan, 2011. Pp. 225–228.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Руцкая, В. И., Тимошенко Е. С. Алкалоиды люпина и способы снижения их содержания. (Обзор). Известия НВ АУК. 2023. 3(71). 573-584. DOI: 10.32786/2071-9485-2023-03-56.</mixed-citation><mixed-citation xml:lang="en">Руцкая, В. И., Тимошенко Е. С. Алкалоиды люпина и способы снижения их содержания. (Обзор). Известия НВ АУК. 2023. 3(71). 573-584. DOI: 10.32786/2071-9485-2023-03-56.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Li X., Yang H., Buirchell B., Yan G. Development of a DNA marker tightly linked to low-alkaloid gene iucundus in narrow-leafed lupin (Lupinus angustifolius L.). // Crop Past Sci, – 2011. – N 62. – P. 218–224.</mixed-citation><mixed-citation xml:lang="en">Li X., Yang H., Buirchell B., Yan G. Development of a DNA marker tightly linked to low-alkaloid gene iucundus in narrow-leafed lupin (Lupinus angustifolius L.). // Crop Past Sci, – 2011. – N 62. – P. 218–224.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Frick, K.M.; Foley, R.C.; Kamphuis, L.G.; Siddique, K.H.M.; Garg, G.; Singh, K.B. Characterization of the genetic factors affecting quinolizidine alkaloid biosynthesis and its response to abiotic stress in narrow-leafed lupin (Lupinus angustifolius L.). Plant Cell Environ. 2018, 41, 2155–2168.</mixed-citation><mixed-citation xml:lang="en">Frick, K.M.; Foley, R.C.; Kamphuis, L.G.; Siddique, K.H.M.; Garg, G.; Singh, K.B. Characterization of the genetic factors affecting quinolizidine alkaloid biosynthesis and its response to abiotic stress in narrow-leafed lupin (Lupinus angustifolius L.). Plant Cell Environ. 2018, 41, 2155–2168.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Cowling, W.A.; Huyghe, C.; Swiecicki, W. Lupin breeding. In Lupins as Crop Plants: Biology, Production and Utilization; Gladstones, J., Atkins, C.A., Hamblin, J., Eds.; CAB International: London, UK, 1998; pp. 93–120.</mixed-citation><mixed-citation xml:lang="en">Cowling, W.A.; Huyghe, C.; Swiecicki, W. Lupin breeding. In Lupins as Crop Plants: Biology, Production and Utilization; Gladstones, J., Atkins, C.A., Hamblin, J., Eds.; CAB International: London, UK, 1998; pp. 93–120.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Abraham E. M. The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches [Electronic resource] / Eleni M. Abraham, I. Ganopoulos, P. Madesis, A. Mavromatis [and others.] https://www.mdpi.</mixed-citation><mixed-citation xml:lang="en">Abraham E. M. The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches [Electronic resource] / Eleni M. Abraham, I. Ganopoulos, P. Madesis, A. Mavromatis [and others.] https://www.mdpi.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Bunsupa, S.; Yamazaki, M.; Saito, K. Quinolizidine alkaloid biosynthesis: Recent advances and future prospects. Front. Plant Sci. 2012, 3, 239.</mixed-citation><mixed-citation xml:lang="en">Bunsupa, S.; Yamazaki, M.; Saito, K. Quinolizidine alkaloid biosynthesis: Recent advances and future prospects. Front. Plant Sci. 2012, 3, 239.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Hane, J.K.; Ming, Y.; Kamphuis, L.G.; Nelson, M.N.; Garg, G.; Atkins, C.A.; Bayer, P.E.; Bravo, A.; Bringans, S.; Cannon, S.; et al. A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: Insights into plant-microbe interactions and legume evolution. Plant Biotechnol. J. 2017, 15, 318–330.</mixed-citation><mixed-citation xml:lang="en">Hane, J.K.; Ming, Y.; Kamphuis, L.G.; Nelson, M.N.; Garg, G.; Atkins, C.A.; Bayer, P.E.; Bravo, A.; Bringans, S.; Cannon, S.; et al. A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: Insights into plant-microbe interactions and legume evolution. Plant Biotechnol. J. 2017, 15, 318–330.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Nelson, M.N.; Moolhuijzen, P.M.; Boersma, J.G.; Chudy, M.; Lesniewska, K.; Bellgard, M.; Oliver, R.P.; Swiecicki, W.; Wolko, B.; Cowling, W.A.; et al. Aligning a new reference genetic map of Lupinus angustifolius with the genome sequence of the model legume, lotus japonicus. DNA Res. 2010, 17, 73–83.</mixed-citation><mixed-citation xml:lang="en">Nelson, M.N.; Moolhuijzen, P.M.; Boersma, J.G.; Chudy, M.; Lesniewska, K.; Bellgard, M.; Oliver, R.P.; Swiecicki, W.; Wolko, B.; Cowling, W.A.; et al. Aligning a new reference genetic map of Lupinus angustifolius with the genome sequence of the model legume, lotus japonicus. DNA Res. 2010, 17, 73–83.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
