EVALUATION OF THE “POD INDEHISCENCE” TRAIT OF NARROW-LEAVED LUPINE (Lupinus angustifolius L.) BREEDING MATERIAL WITH THE USE OF THE MARKER-ASSISTED SELECTION TECHNOLOGY

Abstract

The paper demonstrates the results of the studies on the trait “pod indehiscence” of narrow-leaved lupin (Lupinus angustifolius L.) breeding material as well as the results of the development of the methodology for marker-assisted selection of narrow-leaved lupin with regard to this trait.
An analysis of the DNA typing results shows that all the collection accessions have the identified cultural alleles of the “pod indehiscence” gene lentus. Polymorphism is identified in the gene tardus of the studied collection: about 20% of the accessions have the wild allele, which determines an undesirable trait - spontaneous pod dehiscence during maturation. Genotyping of the obtained breeding samples of narrow-leaved lupine for the gene tardus using the TaLi marker shows that the vast majority of them (over 85%) have the cultural pod indehiscence allele TaLid of 511 bps, 15% of the samples are prone to dehiscence. To exclude undesirable alleles in the breeding process, it’s required to conduct a thorough analysis and monitor this trait applying the methods of molecular genetic analysis. The use of marker analysis combined with phenotypic evaluation of the experimental material of narrow-leaved lupine results in identifying the samples that are attractive for breeding.