IDENTIFICATION OF HMW GLUTENIN POLYMORPHISM IN VARIETY SAMPLES FROM COLLECTION AND BREEDING NURSURIES OF WINTER WHEAT
Abstract
The analysis of polymorphism of such genes as Glu-А1x, Glu-B1x and Glu-B1y, Glu-D1x and Glu-D1y controlling synthesis of storage seed proteins (glutenins) was conducted in 45 variety samples of winter wheat. Probable bread-making qualities of the studied lines were estimated. The test results of Belarusian wheat samples showed that among them, a high share of allels in Glu-А1 and Glu-В1 loci influencing the bread-making qualities negatively, and d allel in Glu-D1 locus contributing positively to flour quality, was observed. As a result of the researches conducted, genotypes containing favourable combinations of the alleles of high-molecular-weight glutenins were isolated. 4 variety samples with the highest indices of breadmaking qualities (10 points) were found out. The lowest overall breadmaking estimate was in the variety samples in which parents were only West-European varieties, such as STH 729, STH 735, Kubus, Kriss, STH 583, STH 1198, Kontur, Kornet, and as well as when Belarusian varieties with low bread-making qualities (Schara and Garmoniya) were used.
About the Authors
E. A. FominaBelarus
S. N. Kulinkovich
Belarus
S. V. Malyshev
Belarus
O. Y. Urbanovich
Belarus
References
1. Кулинкович, С.Н. Озимая пшеница в вопросах и ответах / С.Н. Кулинкович, В.С.Бобер. – Минск: Наша Идея, 2012. – 320 с.
2. Кулинкович, С.Н. Урожайность зерна сортов озимой пшеницы в южном регионе Республики Беларусь / С.Н. Кулинкович, О.А. Барановская, С.В. Кравцов // Земляробства i ахова раслiн. – 2014. – №5. – С. 26-29.
3. Gale, K.R. Diagnostic DNA markers for quality traits in wheat / K.R. Gale // Journal of Cereal Science. – 2005. – Vol. 41, №2. – P. 181-192.
4. Ma, W. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat / W. Ma, W. Zhang, K.R. Gale // Euphytica. – 2003. – Vol. 134. – P. 51-60.
5. Laflandra, D. PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat / D. Laflandra, G.F. Tucci, A. Pavoni, T. Turchetta, B. Margiotta // Theor. Appl. Genet. – 1997. – Vol. 94. – P. 235-240.
6. Ahmad, M. Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers / M. Ahmad // TAG Theoretical and Applied Genetics. – 2000. – Vol. 101, №5-6. – P. 892-896.
7. Liu, S. New DNA markers for high molecular weight glutenin subunits in wheat / S. Liu, S. Chao, J.A. Anderson // Theor Appl Genet. – 2008. – Vol. 118, №1. – P. 177-183.
8. Giroux, M.J. Puroindolines: their role in grain hardness and plant defence / M.J. Giroux [et al.] // Biotechnol. Genet. Eng Rev. – 2003. – Vol. 20. – P. 277-290.
9. Lillemo, M. A leucine to proline mutation in puroindoline b is frequently present in hard wheats from Northern Europe / M. Lillemo, C.F. Morris // Theor. Appl. Genet. – 2000. – Vol. 100, №7. – P. 1100-1107.
10. Korzun, V. Genetic analysis of the dwarfing gene (Rht8) in wheat / Korzun V., Roder M.S., Ganal M.W., Worland A.J., Law C.W. // Theor. Appl. Genet. – 1998. – Vol. 96, №8. – P. 1104-1109. – Part I: Molecular mapping of Rht8 on short arm of chromosome 2D of bread wheat (Triticum aestivum L.).
11. MacRitchie, F. Flour polypeptides related to wheat quality / F. MacRitchie, D.L. Cros, C.W. Wrigley // Advances in cereal science and technology. – 1990. – Vol. 10. – P. 79-145.
12. Lukov, O.M. Screening of bread wheats for milling and baking quality-A Canadian perspective / O.M. Lukov // Cereal Foods World. – 1991. – Vol. 36, №6. –P. 497-501.
13. Dessalegn, T. Allelic variation of HMW glutenin subunits of Ethiopian bread wheat cultivars and their quality / T. Dessalegn, C.S. Van Deventer, M.T. Labuschagne, H. Martens // African Crop Science Journal. – 2011. – Vol. 19, №2. – P. 55-63.
14. Добротворская, Т.В. Анализ разнообразия российских и украинских сортов мягкой пшеницы (Triticum aestivum L.) по высокомолекулярным субъединицам глютенина / Т.В. Добротворская, С.П. Мартынов // Генетика. – 2011. – Т. 47, №7. – С. 905-919.
15. Bradova, J. Evaluation of winter wheat collection in terms of HMW- and LMW-glutenin subunits / J. Bradova, L. Stockova // Czech. J. Genet. Plant Breed. – 2010. – Vol. 46. – P. 596-599.
16. Payne, P.I. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties / P.I. Payne, M.A. Nigtingale, A.F. Krattiger, L.M. Holt / J. Sci. Food Agriculture. – 1987. – Vol. 40. – P. 51-65.
17. Rogers, W.J. The HMW glutenin subunit and gliadin compositions of German-Grown wheat varieties and their relationship with breadmaking quality / W.J. Rogers, P.I. Payne, K. Harinder // Plant Breeding. – 1989. – Vol. 103. – P. 89-100.
Review
For citations:
Fomina E.A., Kulinkovich S.N., Malyshev S.V., Urbanovich O.Y. IDENTIFICATION OF HMW GLUTENIN POLYMORPHISM IN VARIETY SAMPLES FROM COLLECTION AND BREEDING NURSURIES OF WINTER WHEAT. Arable Farming and Plant Breeding in Belarus. 2014;(50):326-335. (In Russ.)