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SCREENING OF BLUE LUPINE ACCESSION COLLECTION FOR THE GENES OF ECONOMICALLY IMPORTANT TRAITS

Abstract

As a result of the conducted research the methodology of marker assisted selection in blue lupine has been tried and tested for the following traits: resistance to anthracnose, pod dehiscence, alkaloid content, tendency to vernalization, moisture permeability of a seed coat. The accessions with economically important traits important for breeding in the Republic of Belarus have been identified. The analysis of the results of DNA-typing shows that all the accessions of the studied collection have alleles of the following genes: iucundus (reduced alkaloid content), Ku (thermoneutrality), mollis (moisture permeability of a seed coat), lentus (pod indehiscence). In the studied collection polymorphism has been identified through the pod indehiscence gene tardus. About 20 % of the accessions possess a wild allele determining an undesirable trait of pod self-dehiscence during the maturation process. Anthracnose resistance genotyping allows identifying accessions with resistance alleles

About the Authors

N. V. Anisimova
Институт генетики и цитологии НАН Беларуси
Belarus


E. N. Sysolyatin
Институт генетики и цитологии НАН Беларуси
Belarus


M. N. Kritsky
РУП «Научно-практический центр НАН Беларуси по земледелию»
Belarus


V. Ch. Shor
РУП «Научно-практический центр НАН Беларуси по земледелию»
Belarus


V. V. Grin
РУП «Научно-практический центр НАН Беларуси по земледелию»
Belarus


A. A. Kozlovsky
РУП «Научно-практический центр НАН Беларуси по земледелию»
Belarus


A. V. Kilchevsky
Институт генетики и цитологии НАН Беларуси
Belarus


References

1. Анисимова, Н.В. Анализ коллекции люпина узколистного (Lupinus angustifolius L.) по генам хозяйственно-ценных признаков / Н.В. Анисимова [и др.] // Земледелие и селекция в Беларуси: сб. науч. тр.; редкол.: Ф.И. Привалов (гл ред.) [и др.] / Научно-практический центр НАН Беларуси по земледелию. – Минск, 2018. – Вып. 54. – С. 300–308.

2. Привалов, Ф.И. Перспективы возделывания, селекции и семеноводства люпина в Беларуси. / Ф.И. Привалов, В.Ч. Шор // Весцi НАН Беларусi. – 2015. – №2. – С. 47–53.

3. Стержневая генетическая коллекция Lupinus angustifolius L.: генетика, формирование биологического банка генов, использование / Н. С. Купцов [и др.]; рец.: С.И. Гриб, В.С. Анохина; НАН Беларуси, РУП «Научно-практический центр НАН Беларуси по земледелию». – Минск, 2014. – 126 с.

4. Application of next-generation sequencing for rapid marker development in molecular plant breeding: a case study on anthracnose disease resistance in Lupinus angustifolius L. / H. Yang [et al.] // BMC Genomics. – 2012. – Vol. 13. – P. 318.

5. Boersma, J.G. et al. Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.) / J.G.Boersma, B.J.Buirchell, K.Sivasithamparam, H.Yang // Genet Mol Biol. – 2007. – Vol. 30. – P. 623–629.

6. Boersma, J.G. et al. Development of a sequence-specific marker linked to the Ku gene which removes the vernalization requirement in narrow-leafed lupin / J.G.Boersma, B.J.Buirchell, K.Sivasithamparam, H.Yang // Plant breed. – 2007. – Vol. 126. – P. 306–309.

7. Li, X. Development of a co-dominant DNA marker tightly linked to gene tardus conferring reduced pod shattering in narrow-leafed lupin (Lupinus angustifolius L.) / X. Li, G. Yan, D. Renshaw, H. Yang // Euphytica, 2010. – N. 176. – P. 49–58.

8. Li, X. Development of a DNA marker tightly linked to low-alkaloid gene iucundus in narrow-leafed lupin (Lupinus angustifolius L.). / X. Li, H. Yang, B. Buirchell, G.Yan // Crop Past Sci, 2011. – N. 62. – P. 218–224.

9. Li, X. et al. A molecular marker linked to the mollis gene conferring soft-seediness for marker-assisted selection applicable to a wide range of crosses in lupin (Lupinus angustifolius L.) breeding / X.Li, B.Buirchell, G.Yan, Yang H. // Mol Breed. – 2012. – Vol. 29. – P. 361–370.

10. Yang, H. et al. A strategy to develop molecular markers applicable to a wide range of crosses for marker assisted selection in plant breeding: a case study on anthracnose disease resistance in lupin (Lupinus angustifolius L.) / H.Yang, D.Renshaw, G.Thomas, B.Buirchell, M.Sweetingham // Mol Breed. – 2008. – Vol. 21. – P. 473–483.


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For citations:


Anisimova N.V., Sysolyatin E.N., Kritsky M.N., Shor V.Ch., Grin V.V., Kozlovsky A.A., Kilchevsky A.V. SCREENING OF BLUE LUPINE ACCESSION COLLECTION FOR THE GENES OF ECONOMICALLY IMPORTANT TRAITS. Arable Farming and Plant Breeding in Belarus. 2020;(56):379-388. (In Russ.)

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ISSN 0130-156X (Print)